ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for determining anticoagulation potency of Sparganii Rhizoma, and evaluate the effect of Sparganii Rhizoma herbs from different producing areas on promoting blood circulation and removing blood stasis; and study the material basis of Sparganii Rhizoma through the correlation analysis on its anticoagulation potency, ferulic acid and total flavonoid content.</p><p><b>METHOD</b>The anticoagulation time of Sparganii Rhizoma from different producing areas with activeated partial thromboplastin time for their active extracts. Their biopotency was calculated by using the method of "parallel lines of dose effect" (3, 3). The degree of correlation between their anticoagulation potency and chemical constituents were calculated by using Pearson correlational analysis method.</p><p><b>RESULT</b>Sparganii Rhizoma and is control drugs had a good linear relationship between dose and effect (Y = 172.76X - 193.39, R2 = 0.9955). The method had better accuracy (RSD 4.7%), repeatability (RSD 2.3%) and intermediate precision (RSD 5.4%), finding that the biopotency of Sparganii Rhizoma from different producing areas ranged between 52.33-238.58 U x g(-1), and all of them passed the test on reliability. The results of correlation analysis showed no remarkable relationship between the anticoagulation potency of Sparganii Rhizoma and the contents of the two chemical constituents.</p><p><b>CONCLUSION</b>This biopotency determination method established in the experiment can be used as one of approaches for qulaity evaluation on Sparganii Rhizoma.</p>
Subject(s)
Animals , Rabbits , Anticoagulants , Chemistry , Pharmacology , Blood Coagulation , Drugs, Chinese Herbal , Chemistry , Pharmacology , Rhizome , Chemistry , Typhaceae , ChemistryABSTRACT
In order to look for lower toxic and strongly active compounds, the structure of sesquiterpene lactones from Elephantopus scaber was modified. Deoxyelephantopin and scabertopin were isolated from the whole plant of Elephantopus scaber and hydrogenated and epoxidated. Five sesquiterpenoide derivatives were prepared and their structures were identified by IR, NMR and MS spectra analysis. Four sesquiterpenoides are new compounds. Part of the compounds show definite antitumor activity.
Subject(s)
Humans , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Asteraceae , Chemistry , HeLa Cells , Hydrogenation , Lactones , Chemistry , Pharmacology , Molecular Structure , Plants, Medicinal , Chemistry , Sesquiterpenes , Chemistry , Pharmacology , Terpenes , Chemistry , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To isolate and elucidate the chemical constituents of the whole plant of Vernonia patula, and to provide samples for biological activity screening.</p><p><b>METHOD</b>The chromatography on silica gel was used and the structures were determined by IR, NMR and MS spectra analysis and physicochemical property comparion.</p><p><b>RESULT</b>Four triterpenoids were isolated and identified as bauerenyl acetate(I), friedelin(II), epifriedelanol(III), 20(30)-taraxastene-3 beta, 21 alpha-diol(IV).</p><p><b>CONCLUSION</b>Compounds I and IV were isolated from genus Vernonia for the first time and compounds II and III were isolated from the whole plant of V. patula for the first time. The four compounds show no inhibitory effect on the growing of PC-12 cells.</p>